Database : HANSEN Search on : ESTUDOS TRANSVERSAIS [Subject descriptor] References found : 12 [refine] Displaying: 1 .. 12   in format [Detailed]

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Id: 17554 Author: Young, Saroj K; Taylor, G. Michael; Jain, Suman; Suneetha, Lavanya M; Suneetha, Sujai; Lockwood, Diana N. J; Young, Douglas B Title: Microsatellite mapping of Mycobacterium leprae populations in infected humans ..- Source: s.l; s.n; Nov. 2004. 6 p. ilus, mapas, tab. Abstract: To investigate genetic diversity in a bacterial population, we measured the copy numbers of simple sequence repeats, or microsatellites, in Mycobacterium leprae from patients living in and around Hyderabad, India. Three microsatellite loci containing trinucleotide or dinucleotide repeats were amplified from infected tissues, and the copy numbers were established by sequence analysis. Extensive diversity was observed in a cross-sectional survey of 33 patients, but closely related profiles were found for members of a multicase family likely to share a common transmission source. Sampling of multiple tissues from single individuals demonstrated identical microsatellite profiles in the skin, nasal cavity, and bloodstream but revealed differences at one or more loci for M. leprae present in nerves. Microsatellite mapping of M. leprae represents a useful tool for tracking short transmission chains. Comparison of skin and nerve lesions suggests that the evolution of disease within an individual involves the expansion of multiple distinct subpopulations of M. leprae. (AU). Descriptors: Técnica de Tipagem Bacteriana/* Estudos Transversais Família Dosagem de Genes Hanseníase/EP/*MI/*TM Repetições de Microssatélites/*GE Mycobacterium leprae/*CL/*GE Reação em Cadeia da Polimerase Especificidade de Espécies Variação (Genética)/* Limits: HUMANO MASCULINO FEMININO SUPPORT, NON-U.S. GOV'T Location: BR191.1; 09333/s

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Id: 13643 Author: Guerrero, Martha Inírida; Arias, María Teresa; Garcés, María Teresa; León, Clara Inés Title: Desarrollo y aplicación de una prueba de RCP para detectar la infección subclínica por Mycobacterium leprae Developing and using a PCR test to detect subclinical Mycobacterium leprae infection- Source: s.l; s.n; 2002. 7 p. ilus, tab. Abstract: OBJECTIVE: While the prevalence of leprosy has declined around the world, there has not been a corresponding decrease in its incidence, thus indicating that it has not been possible to prevent transmission of the disease. Despite the small number of patients with lepromatous leprosy, the majority of the inhabitants of endemic areas show signs of exposure to Mycobacterium leprae, which could be explained by the presence of subclinical bacilliferous infections in the community. The objective of this study was to investigate the use of a polymerase chain reaction (PCR) test to detect M. leprae in samples of nasal mucus from asymptomatic household contacts of patients with leprosy. METHODS: We standardized and optimized a PCR technique to amplify a 321 base pair DNA fragment, using a pair of primers complementary to a segment of an LSR/A15 gene that codes for the 15 kDa M. leprae antigen. We investigated the optimal concentrations of all the test components. We used dimethyl sulfoxide (DMSO) to achieve a more specific amplification. We applied the PCR test to 70 healthy household contacts of leprosy patients from eight municipalities in Colombia where there was a high prevalence of the disease. RESULTS: The test's detection limit was 100 fg of DNA. With the optimized technique, bacillus was detected in the nasal mucus samples of 9 (12.8 per cent) of the 70 household contacts. The 3 PCR-positive household contacts of paucibacillary cases were from municipalities with very high prevalence levels. In comparison to contacts who were PCR-negative, the contacts who were PCR-positive had spent significantly less time, as a proportion of their age, living with a patient (P = 0.028). This finding demonstrates the test's capacity for early detection. CONCLUSIONS: The PCR test that we developed is useful as a tool for detection and early follow-up of possible leprosy cases. It can be used to monitor high-risk populations and also to maintain the achievements of leprosy elimination programs in countries where the disease's prevalence has been significantly reduced. (AU). Descriptors: ESTUDOS TRANSVERSAIS HANSENIASE/diag HANSENIASE/epidemiol MYCOBACTERIUM LEPRAE REACAO EM CADEIA DA POLIMERASE/métodos Limits: HUMANO MASCULINO FEMININO ADULTO SUPPORT, NON-U.S. GOV'T RESUMO EM INGLES Location: BR191.1; 09066/s

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Id: 12239 Author: Keita, S; Tiendrebeogo, A; Konare, H; Cisse, B.-S; Faye, O Title: Nouveaux cas de lèpre à L'Institut Marchoux (Bamako, Mali): Etude comparative 1988-1997 New cases of leprosy at the Marchoux Institute: a comparative study 1988-1997- Source: s.l; s.n; 2001. 3 p. tab. Descriptors: CRIANÇA ESTUDOS TRANSVERSAIS HANSENIASE MALI ESTUDOS RETROSPECTIVOS ESTUDOS PROSPECTIVOS Limits: ESTUDO COMPARATIVO Location: BR191.1; 08641/s

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Id: 11661 Author: Prasad, P. V. S Title: Childhood leprosy in a rural hospital ..- Source: s.l; s.n; 1998. 4 p. tab. Descriptors: ADOLESCENCIA CRIANÇA PRÉ-ESCOLAR ESTUDOS TRANSVERSAIS HOSPITAIS RURAIS INCIDENCIA INDIA LACTENTE HANSENIASE HANSENIASE POPULAÇAO RURAL Location: BR191.1; 08538/s

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Id: 10348 Author: Anon Title: Progress towards the elimination of leprosy as a public health problem ..- Source: s.l; s.n; 1995. 6 p. tab. Descriptors: COMPARAÇAO TRANSCULTURAL ESTUDOS TRANSVERSAIS PREVISOES INCIDENCIA HANSENIASE HANSENIASE SAUDE PUBLICA ORGANIZAÇAO MUNDIAL DA SAUDE Limits: ESTUDO COMPARATIVO Location: BR191.1; 07200/s

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Id: 10346 Author: Anon Title: Leprosy disabilities: magnitude of the problem ..- Source: s.l; s.n; 1995. 7 p. tab, graf. Descriptors: ADOLESCENCIA CRIANÇA PRÉ-ESCOLAR ESTUDOS TRANSVERSAIS PAISES EM DESENVOLVIMENTO AVALIAÇAO DA INCAPACIDADE DEFICIENTES QUIMIOTERAPIA COMBINADA INCIDENCIA LACTENTE LEPROSTATICOS LEPROSTATICOS HANSENIASE HANSENIASE ESPERANÇA DE VIDA ORGANIZAÇAO MUNDIAL DA SAUDE Location: BR191.1; 07197/s

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Id: 9978 Author: Ulrich, Marian; Zulueta, Ana Maria; Caceres-Dittmar, Gisela; Sampson, Celsa; Pinardi, Maria Eugenia; Rada, Elsa Maria; Aranzazu, Nacarid Title: Leprosy in women: characteristics and repercussions ..- Source: s.l; s.n; 1993. 12 p. tab, graf. Descriptors: ESTUDOS TRANSVERSAIS PAISES EM DESENVOLVIMENTO AVALIAÇAO DA INCAPACIDADE EDUCAÇAO EM SAUDE INCIDENCIA HANSENIASE HANSENIASE HANSENIASE HANSENIASE ACEITAÇAO SOCIAL FATORES SOCIO-ECONOMICOS Location: BR191.1; 06622/s

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Id: 9969 Author: Anon Title: Progress towards the elimination of leprosy as a public health problem ..- Source: s.l; s.n; 1993. 6 p. ilus, tab, mapas. Descriptors: CONTROLE DE DOENÇAS TRANSMISSIVEIS COMPARAÇAO TRANSCULTURAL ESTUDOS TRANSVERSAIS PAISES EM DESENVOLVIMENTO QUIMIOTERAPIA COMBINADA INCIDENCIA LEPROSTATICOS HANSENIASE HANSENIASE ORGANIZAÇAO MUNDIAL DA SAUDE Location: BR191.1; 06613/s

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Id: 9902 Author: Frommel, Dominique; Tekle-Haimanot, Redda; Verdier, Mireille; Negesse, Yohannes; Bulto, Tesfaye; Denis, Francois Title: HIV infection and leprosy: a four-year survey in Ethiopia ..- Source: s.l; s.n; 1994. 2 p. tab. Descriptors: ESTUDOS TRANSVERSAIS ETIOPIA INFECÇOES POR HIV INFECÇOES POR HIV HIV-1 HANSENIASE HANSENIASE MORBIDADE Location: BR191.1; 06889/s

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Id: 9687 Author: Frommel, Dominique; Tekle-Haimanot, Redda; Berhe, Nega; Aussel, Laure; Verdier, Mireille; Preux, Pierre-Marie; Denis, Francois Title: A survey of antibodies to hepatitis C virus in Ethiopia ..- Source: s.l; s.n; 1993. 5 p. tab. Descriptors: ADOLESCENCIA FATORES ETARIOS CRIANÇA ESTUDOS DE COORTES ESTUDOS TRANSVERSAIS ETIOPIA ANTICORPOS ANTI-HEPATITE HEPATITE C HEPATITE C ANTICORPOS PRESENTES EM HEPATITE C TÉCNICAS IMUNOENZIMATICAS HANSENIASE DOENÇAS DO SISTEMA NERVOSO PREVALENCIA FATORES DE RISCO POPULAÇAO RURAL DERMATOPATIAS HEPACIVIRUS/*IM DERMATOPATIAS/CO Location: BR191.1; 06663/s

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Id: 9327 Author: Kumar, Dinesh B; Prasad, Eshwar C; Krishnaswamy, Kamala Title: Detection of rifampicin-induced nephrotoxicity by N-acetyl-3-D-glucosaminidase activity ..- Source: s.l; s.n; 1992. 4 p. graf. Descriptors: HANSENIASE ANTIBIOTICOS COMBINADOS ANTIBIOTICOS COMBINADOS ESTUDOS TRANSVERSAIS SINERGISMO DE DROGAS TAXA DE FILTRAÇAO GLOMERULAR RIM RIFAMPINA RIFAMPINA ESTREPTOMICINA ESTREPTOMICINA TUBERCULOSE PULMONAR Limits: HUMANO MEIA-IDADE Location: BR191.1; 06474/s

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Id: 3611 Author: Pfau, Ruth Title: Analysis of leprosy control programme in Pakistan ..- Source: s.l; s.n; 1984. 13 p. tab. Descriptors: HANSENIASE TERAPIA COMBINADA ESTUDOS TRANSVERSAIS Limits: HUMANO Location: BR191.1; 02449/s

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